A label-free double-amplification system for sensitive detection of single-stranded DNA and thrombin by liquid chromatography-mass spectrometry.

نویسندگان

  • Wenbo Zhao
  • Zhen Qin
  • Chengsen Zhang
  • Meiping Zhao
  • Hai Luo
چکیده

A label-free double amplification system has been developed by using a ternary DNA probe containing the poly(adenine-thymine) sequence assisted by exonuclease III degradation. The method achieved more than 600-fold signal amplification and allowed sensitive detection of single-stranded DNA and thrombin at the pM level by using liquid chromatography/mass spectrometry.

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Supporting Information A label-free double-amplification system for sensitive detection of single-stranded DNA and thrombin by liquid chromatography-mass spectrometry

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عنوان ژورنال:
  • Chemical communications

دوره 50 69  شماره 

صفحات  -

تاریخ انتشار 2014